Biovision Also Has A Fluorogenic Ptp Assay Kit

Description: The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.

Description: The Fluorogenic HDAC9 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 9 (HDAC9) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC9 activity measurements. In addition, the kit includes purified_x000D_HDAC9 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC9 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC9 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC9. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC3 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 3 (HDAC3) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified_x000D_HDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC3 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader.

Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_ HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_ substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The Fluorogenic MALT1 Assay Kit is designed to measure MALT1 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Description: The Fluorogenic MMP2 Assay Kit is designed to measure MMP2 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Description: The Fluorogenic MMP8 Assay Kit is designed to measure MMP8 activity for screening and profiling applications, in a homogeneous assay with no timeconsuming washing steps. 

Description: The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.

Description: The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_