Biovision Also Has A Fluorogenic Ptp Assay Kit

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The A20 Fluorogenic Assay Kit is designed to measure A20 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC2 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 2 (HDAC2) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified_x000D_HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC2 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC2. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader.

Description: The Fluorogenic HDAC4 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 4 (HDAC4) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC4 activity measurements. In addition, the kit includes purified_x000D_HDAC4 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC4 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC4 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC4. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D__x000D_

Description: The Fluorogenic HDAC5 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 5 (HDAC5) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC5 activity measurements. In addition, the kit includes purified_x000D_HDAC5 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC5 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC5 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC5. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC7 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 7 (HDAC7) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC7 activity measurements. In addition, the kit includes purified_x000D_HDAC7 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC7 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC7 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC7. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.

Description: The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_

Description: The Fluorogenic MMP9 (Q279R) Assay Kit is designed to measure MMP9 (Q279R) activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate

Description: Protein phosphorylation is one of the most important post-translational modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of insulin receptor kinases and plays a critical role in insulin signaling. The Fluorogenic PTP1B (Catalytic Region) Assay Kit is designed to inhibitors of the catalytic region of PTP1B in a homogeneous assay with no time-consuming washing steps. Note: To identify inhibitors of the regulatory region of PTP1B, please use our Fluorogenic PTP1B (Full Length) Assay Kit, #79766).

Description: The Fluorogenic SIRT1 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling_x000D_applications. The Fluorogenic SIRT1_x000D_ Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_ This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_ steps on a microtiter plate are needed to analyze the SIRT1 activity level. First,_x000D_ the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated_x000D_ lysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation_x000D_ sensitizes the substrate so subsequent treatment with the Lysine Developer_x000D_ produces a fluorophore that can then be measured using a fluorescence reader.

Description: The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure_x000D_ SIRT5 activity for screening and profiling applications. The Fluorogenic_x000D_ SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_ This assay method eliminates dealing with the radioactivity, extraction, and_x000D_ chromatography aspects of traditional assays. Using this kit, only three simple steps on_x000D_a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5_x000D_ fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is_x000D_ incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The Fluorogenic SIRT2 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents_x000D_ necessary for 32 fluorescent SIRT2 activity measurements*.The kit_x000D_ includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a_x000D_ positive and negative control, respectively. The Fluorogenic SIRT2 Assay Kit is_x000D_ based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography_x000D_ aspects of traditional assays. Using this kit, only two simple steps on a microtiter_x000D_plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric_x000D_ substrate (SIRT substrate 1), containing an acetylated lysine side chain, is_x000D_incubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so_x000D_ subsequent treatment with the Lysine Developer produces a fluorophore that can_x000D_then be measured using a fluorescence reader._x000D_

Description: The Fluorogenic SIRT3 Assay Kit is a complete assay system_x000D_designed to measure Sirtuin 3 (SIRT3) activity for screening and profiling_x000D_applications. The Fluorogenic SIRT3 Assay Kit is based on_x000D_a unique fluorogenic substrate and developer combination. This assay method_x000D_eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are_x000D_needed to analyze the SIRT3 activity level. First, the HDAC fluorometric_x000D_substrate (HDAC substrate 1), containing an acetylated lysine side chain, is_x000D_incubated with purified SIRT3 enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can_x000D_then be measured using a fluorescence reader.