Biovision Also Has A Fluorogenic Ptp Assay Kit

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_

Description: The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.

Description: The Fluorogenic MMP11 Assay Kit is designed to measure MMP11 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The A20 Fluorogenic Assay Kit is designed to measure A20 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The Fluorogenic MALT1 Assay Kit is designed to measure MALT1 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Description: The Fluorogenic MMP2 Assay Kit is designed to measure MMP2 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Description: The Fluorogenic MMP8 Assay Kit is designed to measure MMP8 activity for screening and profiling applications, in a homogeneous assay with no timeconsuming washing steps. 

Description: The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_

Description: The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.