Genome-wide evaluation within the mouse embryo reveals the significance of DNA methylation for transcription integrity
Mouse embryos purchase international DNA methylation of their genome throughout implantation. Nevertheless the precise roles of DNA methyltransferases (DNMTs) in embryos haven’t been studied comprehensively. Right here we systematically analyze the implications of genetic inactivation of Dnmt1, Dnmt3a and Dnmt3b on the methylome and transcriptome of mouse embryos.
We discover a strict division of operate between DNMT1, liable for upkeep methylation, and DNMT3A/B, solely liable for methylation acquisition in improvement. By analyzing severely hypomethylated embryos, we uncover a number of capabilities of DNA methylation that’s used as a mechanism of repression for a panel of genes together with not solely imprinted and germline genes, but additionally lineage-committed genes and 2-cell genes.
DNA methylation additionally suppresses a number of retrotransposons and illegitimate transcripts from cryptic promoters in transposons and gene our bodies. Our work supplies a radical evaluation of the roles of DNA methyltransferases and the significance of DNA methylation for transcriptome integrity in mammalian embryos.
Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
Transcription components (TFs) regulate goal genes by particular interactions with DNA sequences. Detecting and understanding these interactions on the molecular degree is of basic significance in organic and medical contexts. Crosslinking mass spectrometry is a robust software to help the construction prediction of protein complexes however has been restricted to the examine of protein-protein and protein-RNA interactions.
Right here, we current a femtosecond laser-induced crosslinking mass spectrometry (fliX-MS) workflow, which permits the mapping of protein-DNA contacts at single nucleotide and as much as single amino acid decision. Utilized to recombinant histone octamers, NF1, and TBP in complicated with DNA, our methodology is extremely particular for the mapping of DNA binding domains.
Recognized crosslinks are in shut settlement with earlier biochemical information on DNA binding and largely match recognized complicated buildings. Making use of fliX-MS to cells identifies a number of bona fide crosslinks on DNA binding domains, paving the way in which for future massive scale ex vivo experiments.
Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
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| VAng-Cr6538-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4893.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
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Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
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| VAng-Cr6538-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3459.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
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Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
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| VAng-Cr6538-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2370 |
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Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
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Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
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| VAng-Cr5594-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5602.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
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Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
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| VAng-Cr5594-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3969.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
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Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
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| VAng-Cr5594-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2700 |
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Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
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Recombinant Staphylococcus Aureus araB Protein (aa 1-545) (strain MSSA476) |
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| VAng-Cr5613-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) Ribulokinase (araB), recombinant protein. |
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Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
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| VAng-Cr5668-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5157.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
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Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
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| VAng-Cr5668-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3657.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
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Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
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| VAng-Cr5668-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2502 |
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Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
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Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
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| VAng-Cr5684-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3195.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
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Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
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| VAng-Cr5684-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2271.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
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Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
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| VAng-Cr5684-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1578 |
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Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
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Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
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| VAng-Cr5686-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5784 |
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Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
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Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
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| VAng-Cr5686-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4084.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
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Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
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| VAng-Cr5686-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2782.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
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Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
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| VAng-Cr5693-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3820.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
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Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
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| VAng-Cr5693-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2733.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
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Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
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| VAng-Cr5693-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1875.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
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Recombinant Staphylococcus Aureus dnaG Protein (aa 1-605) (strain MSSA476) |
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| VAng-Cr5703-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) DNA primase (dnaG), recombinant protein. |
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Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
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| VAng-Cr5740-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4926 |
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Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
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Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
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| VAng-Cr5740-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3490.8 |
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Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
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Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
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| VAng-Cr5740-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2386.8 |
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Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
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Recombinant Staphylococcus Aureus flp Protein (aa 1-498) (strain MSSA476) |
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| VAng-Cr5760-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) Protein flp (flp), recombinant protein. |
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Recombinant Staphylococcus Aureus fnbA Protein (aa 1-1015) (strain MSSA476) |
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| VAng-Cr5767-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) Fibronectin-binding protein A (fnbA), recombinant protein. |
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Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
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| VAng-Cr5775-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6213.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
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Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
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| VAng-Cr5775-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4398 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
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Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
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| VAng-Cr5775-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2997.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
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Recombinant Staphylococcus Aureus ftsK Protein (aa 1-789) (strain MSSA476) |
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| VAng-Cr5778-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) DNA translocase FtsK (ftsK), recombinant protein. |
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Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
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| VAng-Cr5784-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4794 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
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Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
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| VAng-Cr5784-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3393.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
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Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
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| VAng-Cr5784-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2320.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
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Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
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| VAng-Cr5811-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2865.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
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Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
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| VAng-Cr5811-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2040 |
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Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
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Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
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| VAng-Cr5811-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1429.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
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Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
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| VAng-Cr5827-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5008.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
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Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
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| VAng-Cr5827-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3540 |
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Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
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Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
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| VAng-Cr5827-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2419.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
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Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
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| VAng-Cr5848-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4216.8 |
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Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
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Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
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| VAng-Cr5848-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3013.2 |
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Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
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Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
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| VAng-Cr5848-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2056.8 |
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Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
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Recombinant Staphylococcus Aureus lrgB Protein (aa 1-233) (strain MSSA476) |
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| VAng-Cr5869-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Staphylococcus Aureus (strain MSSA476) Antiholin-like protein LrgB (lrgB), recombinant protein. |
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Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
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| VAng-Cr5910-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4464 |
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Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
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Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
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| VAng-Cr5910-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3195.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
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Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
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| VAng-Cr5910-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2172 |
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Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
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Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
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| VAng-Cr6005-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3657.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
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Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
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| VAng-Cr6005-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2617.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
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Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
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| VAng-Cr6005-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1809.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
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Recombinant Staphylococcus Aureus panD Protein (aa 1-24) (strain MSSA476) |
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| VAng-Cr6028-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2848.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Aspartate 1-decarboxylase (panD), recombinant protein. |
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Recombinant Staphylococcus Aureus panD Protein (aa 1-24) (strain MSSA476) |
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| VAng-Cr6028-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2040 |
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Description: Staphylococcus Aureus (strain MSSA476) Aspartate 1-decarboxylase (panD), recombinant protein. |
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Recombinant Staphylococcus Aureus panD Protein (aa 1-24) (strain MSSA476) |
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| VAng-Cr6028-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1429.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Aspartate 1-decarboxylase (panD), recombinant protein. |
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Recombinant Staphylococcus Aureus pgk Protein (aa 1-396) (strain MSSA476) |
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| VAng-Cr6041-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5652 |
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Description: Staphylococcus Aureus (strain MSSA476) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Staphylococcus Aureus pgk Protein (aa 1-396) (strain MSSA476) |
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| VAng-Cr6041-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4002 |
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Description: Staphylococcus Aureus (strain MSSA476) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Staphylococcus Aureus pgk Protein (aa 1-396) (strain MSSA476) |
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| VAng-Cr6041-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2733.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Staphylococcus Aureus recR Protein (aa 1-198) (strain MSSA476) |
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| VAng-Cr6120-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4150.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Recombination protein RecR (recR), recombinant protein. |
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Recombinant Staphylococcus Aureus recR Protein (aa 1-198) (strain MSSA476) |
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| VAng-Cr6120-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2964 |
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Description: Staphylococcus Aureus (strain MSSA476) Recombination protein RecR (recR), recombinant protein. |
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Recombinant Staphylococcus Aureus recR Protein (aa 1-198) (strain MSSA476) |
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| VAng-Cr6120-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2023.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Recombination protein RecR (recR), recombinant protein. |
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Recombinant Staphylococcus Aureus sak Protein (aa 28-163) (strain MSSA476) |
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| VAng-Cr6239-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3688.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Staphylokinase (sak), recombinant protein. |
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Recombinant Staphylococcus Aureus sak Protein (aa 28-163) (strain MSSA476) |
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| VAng-Cr6239-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2634 |
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Description: Staphylococcus Aureus (strain MSSA476) Staphylokinase (sak), recombinant protein. |
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Recombinant Staphylococcus Aureus sak Protein (aa 28-163) (strain MSSA476) |
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| VAng-Cr6239-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1809.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Staphylokinase (sak), recombinant protein. |
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Recombinant Staphylococcus Aureus sbi Protein (aa 30-437) (strain MSSA476) |
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| VAng-Cr6418-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5751.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Immunoglobulin-binding protein sbi (sbi), recombinant protein. |
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Recombinant Staphylococcus Aureus sbi Protein (aa 30-437) (strain MSSA476) |
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| VAng-Cr6418-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4068 |
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Description: Staphylococcus Aureus (strain MSSA476) Immunoglobulin-binding protein sbi (sbi), recombinant protein. |
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Recombinant Staphylococcus Aureus sbi Protein (aa 30-437) (strain MSSA476) |
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| VAng-Cr6418-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2766 |
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Description: Staphylococcus Aureus (strain MSSA476) Immunoglobulin-binding protein sbi (sbi), recombinant protein. |
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Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
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| VAng-Cr6433-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4068 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
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Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
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| VAng-Cr6433-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2914.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
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Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
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| VAng-Cr6433-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1990.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
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Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
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| VAng-Cr6446-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4184.4 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
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Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
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| VAng-Cr6446-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
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Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
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| VAng-Cr6446-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
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Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
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| VAng-Cr6449-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4184.4 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
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Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
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| VAng-Cr6449-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
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Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
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| VAng-Cr6449-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
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Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
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Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
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| VAng-Cr6472-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4167.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
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Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
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| VAng-Cr6472-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2980.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
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Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
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| VAng-Cr6472-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2023.2 |
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Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
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Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
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| VAng-Cr6497-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3789.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
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Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
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| VAng-Cr6497-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2716.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
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Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
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| VAng-Cr6497-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1858.8 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
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Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
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| VAng-Cr6500-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4398 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
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Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
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| VAng-Cr6500-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3129.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
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Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
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| VAng-Cr6500-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2139.6 |
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Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
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The replication equipment of LUCA: frequent origin of DNA replication and transcription
Origin of DNA replication is an enigma as a result of the replicative DNA polymerases (DNAPs) aren’t homologous among the many three domains of life, Micro organism, Archaea, and Eukarya. The homology between the archaeal replicative DNAP (PolD) and the big subunits of the common RNA polymerase (RNAP) liable for transcription suggests a parsimonious evolutionary state of affairs.
Beneath this mannequin, RNAPs and replicative DNAPs developed from a typical ancestor that functioned as an RNA-dependent RNA polymerase within the RNA-protein world that predated the arrival of DNA replication. The replicative DNAP of the Final Common Mobile Ancestor (LUCA) can be the ancestor of the archaeal PolD.
Phosphorylation of mitochondrial transcription issue B2 controls mitochondrial DNA binding and transcription
Mammalian cells comprise genetic data in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression have to be coordinated with nuclear gene expression to reply to mobile energetic wants. To realize perception into the coordination between the nucleus and mitochondria, there’s a want to grasp the regulation of transcription of mitochondrial DNA (mtDNA).
Reversible protein post-translational modifications of the mtDNA transcriptional equipment could also be one solution to management mtDNA transcription. Right here we give attention to a member of the mtDNA transcription initiation complicated, mitochondrial transcription issue B2 (TFB2M). TFB2M melts mtDNA on the promoter to permit the RNA polymerase (POLRMT) to entry the DNA template and provoke transcription.
Three phosphorylation websites have been beforehand recognized on TFB2M by mass spectrometry: threonine 184, serine 197, and threonine 313. Phosphomimetics have been established at these positions. Proteins have been purified and analyzed for his or her capacity to bind mtDNA and provoke transcription in vitro.
Our outcomes point out phosphorylation at threonine 184 and threonine 313 impairs promoter binding and prevents transcription. These findings present a possible regulatory mechanism of mtDNA transcription and assist make clear the significance of protein post-translational modifications in mitochondrial operate.
1 Kb DNA Ladder |
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| DL006S | Geneaid Biotech | each | EUR 1.2 |
1 kb DNA Ladder |
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| MBS9143981-05mL | MyBiosource | 0.5mL | EUR 200 |
1 kb DNA Ladder |
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| MBS9143981-25mL | MyBiosource | 2.5mL | EUR 450 |
1 kb DNA Ladder |
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| MBS9143981-5x25mL | MyBiosource | 5x2.5mL | EUR 1840 |
Apex 1 Kb DNA Ladder |
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| 19-115 | Genesee Scientific | 1 ml/Unit | EUR 110.22 |
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Description: 200 Gel Lanes, 1kb - 15kb |
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Geliteâ„¢ 1 kb DNA Ladder |
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| 17756 | AAT Bioquest | 0.5mL | EUR 97 |
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Description: The Geliteâ„¢ 1 kb DNA Ladder is designed for sizing and estimating the mass of double-stranded DNA and PCR products within the 250 to 10,000 bp range on agarose or polyacrylamide gels. |
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PerfectTM 1 kb DNA Ladder |
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| E3130-01 | EURx | 100ug | EUR 43.6 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 500 to 10000 bp(500, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000 bp) |
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PerfectTM 1 kb DNA Ladder |
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| E3130-02 | EURx | 500ug | EUR 172.22 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 500 to 10000 bp(500, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000 bp) |
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BriteRuler? 1 kb DNA Ladder |
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| 9301-100 | Biovision | each | EUR 314.4 |
Fluorescent 1 kb DNA Ladder |
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| M-237L | Jena Bioscience GmbH | 5 x 500µl | EUR 207.7 |
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Description: ready-to-use, blue / blue, green fluorescent |
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Fluorescent 1 kb DNA Ladder |
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| M-237S | Jena Bioscience GmbH | 500µl | EUR 52 |
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Description: ready-to-use, blue / blue, green fluorescent |
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1 Kb Ladder DNA Marker |
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| FYD001-500UL | Yeastern Biotech | 20 µg/ 500 µl | Ask for price |
1 kb DNA Ladder 100bp-10000bp |
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| EDL105-250ul | ELK Biotech | 250ul | EUR 11 |
1 kb DNA Ladder 100bp-10000bp |
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| EDL105-500ul | ELK Biotech | 500ul | EUR 20 |
Wide range 1 kb DNA Ladder |
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| M1191-500 | Biovision | each | EUR 333.6 |
1 KB DNA LADDER, 500UL PER KIT |
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| M-DNA-1KB | CORNING | 1/pk | EUR 84 |
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Description: Bioscience Mol Bio; DNA Ladder |
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6 kb plus DNA Ladder |
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| RM19006 | Abclonal | 500μL | EUR 102 |
6 kb plus DNA Ladder |
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| MBS9143982-05mL | MyBiosource | 0.5mL | EUR 160 |
6 kb plus DNA Ladder |
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| MBS9143982-25mL | MyBiosource | 2.5mL | EUR 285 |
6 kb plus DNA Ladder |
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| MBS9143982-5x25mL | MyBiosource | 5x2.5mL | EUR 1100 |
ReadiUseâ„¢ 1 Kb Plus DNA Ladder |
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| 60050-100ul | AAT Bioquest | 100 ul | EUR 99 |
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Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
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ReadiUseâ„¢ 1 Kb Plus DNA Ladder |
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| 60051-2X250ul | AAT Bioquest | 2X250 ul | EUR 203 |
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Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
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ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder |
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| 60055-100ul | AAT Bioquest | 100 ul | EUR 79 |
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Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
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ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder |
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| 60056-2X250ul | AAT Bioquest | 2X250 ul | EUR 151 |
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Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
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Geliteâ„¢ 100 bp-1 kb DNA Ladder |
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| 17760 | AAT Bioquest | 0.5mL | EUR 97 |
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Description: The Geliteâ„¢ 100 bp-1 kb DNA Ladder is designed to help size and estimate the mass of double-stranded DNA and PCR products within the 100 to 10,000 base pair range on agarose or polyacrylamide gels. |
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1 kb DNA Ladder 500ul per kit - EACH |
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| AXY1016 | Scientific Laboratory Supplies | EACH | EUR 167.4 |
Ready-to-Use 1 KB DNA Ladder |
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| 31022-1 | Biotium | EA | EUR 94 |
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
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| AZ-1105-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
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| AZ-1101 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
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| AZ-1105 | Real Time Primers | 500 Lanes | EUR 277.2 |
50 kb large-Range DNA ladder |
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| 308-025 | GeneOn | 250 µg | EUR 144 |
Perfect PlusM 2 kb DNA Ladder |
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| E3140-01 | EURx | 100loadings | EUR 66.49 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 50 - 2000 bp(50, 150, 300, 500, 750, 1000, 1500, 2000 bp) |
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Perfect PlusM 2 kb DNA Ladder |
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| E3140-02 | EURx | 500loadings | EUR 263.78 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 50 - 2000 bp(50, 150, 300, 500, 750, 1000, 1500, 2000 bp) |
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1 kb Ladder |
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| MBS433527-005mg | MyBiosource | 0.05mg | EUR 160 |
1 kb Ladder |
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| MBS433527-5x005mg | MyBiosource | 5x0.05mg | EUR 580 |
Perfect Plus™M 1 kb DNA Ladder |
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| E3131-01 | EURx | 100ug | EUR 46.87 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 250 to 10000 bp(250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000 bp) |
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Perfect Plus™M 1 kb DNA Ladder |
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| E3131-02 | EURx | 500ug | EUR 187.48 |
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Description: Ready to Use DNA ladder for sizing DNA fragments from 250 to 10000 bp(250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000 bp) |
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1 kb DNA Ladder + 6× Loading Dye (100 lanes) |
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| BRBR0800101 | Westburg | each | EUR 77.39 |
DNA LADDER (100bp) |
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| IB01301 | IBI Scientific | 50uG/500uL | EUR 59.65 |
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Description: [FR] |
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DNA LADDER (100bp) |
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| IB01302 | IBI Scientific | 50uG/500uL | EUR 56.1 |
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Description: (NO LOADING DYE) [FR] |
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DNA LADDER (1KB) |
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| IB01351 | IBI Scientific | 50uG/500uL | EUR 61.65 |
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Description: [FR] |
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100bp DNA ladder |
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| 9K-004-0002 | Bio Basic | 0.5ml | EUR 260.98 |
100bp DNA ladder |
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| TBS4030-0.5mL | Tribioscience | 0.5 ml | EUR 60 |
100bp DNA ladder |
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| TBS4030-1.0mL | Tribioscience | 1.0 ml | EUR 110 |
ACTGene? DNA Marker, 1 kb Ladder, 13 Fragments, 100 loads |
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| ACT-IDMWD1 | ACTGene | each | EUR 178.8 |
VC1kb DNA Ladder, 50µg |
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| NL1409 | Vivantis | each | Ask for price |
DNA Ladder Extra-Large Size (250 bp to 25 kb) |
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| MBS433531-005mg | MyBiosource | 0.05mg | EUR 200 |
DNA Ladder Extra-Large Size (250 bp to 25 kb) |
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| MBS433531-5x005mg | MyBiosource | 5x0.05mg | EUR 755 |
VC100bp DNA Ladder, 50µg |
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| NL1401 | Vivantis | each | Ask for price |
OneMark B DNA Ladder (ready-to-use, 250-1000 kb) |
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| DM110-0100 | GeneOn | 600 µl | EUR 75.6 |
100 bp DNA Ladder |
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| M-214L | Jena Bioscience GmbH | 5 x 500µl | EUR 240.4 |
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Description: ready-to-use, orange / blue |
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100 bp DNA Ladder |
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| M-214S | Jena Bioscience GmbH | 500µl | EUR 60.1 |
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Description: ready-to-use, orange / blue |
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200 bp DNA Ladder |
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| M-215L | Jena Bioscience GmbH | 5 x 500µl | EUR 230.7 |
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Description: ready-to-use, blue / blue |
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200 bp DNA Ladder |
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| M-215S | Jena Bioscience GmbH | 500µl | EUR 57.6 |
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Description: ready-to-use, blue / blue |
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500 bp DNA Ladder |
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| M-216L | Jena Bioscience GmbH | 5 x 500µl | EUR 172.8 |
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Description: ready-to-use, blue / blue |
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500 bp DNA Ladder |
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| M-216S | Jena Bioscience GmbH | 500µl | EUR 43.2 |
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Description: ready-to-use, blue / blue |
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100 bp DNA Ladder |
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| MD104-01 | Vazyme | 250 μl | EUR 18.64 |
100 bp DNA Ladder |
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| MD104-02 | Vazyme | 500 μl | EUR 29 |
100 bp DNA Ladder |
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| M1158-500 | Biovision | each | EUR 333.6 |
100 bp DNA Ladder |
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| RM19007 | Abclonal | 500μL | EUR 138 |
100 bp DNA Ladder |
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| DL007 | Geneaid Biotech | each | EUR 17.3 |
100 bp DNA Ladder |
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| DL007S | Geneaid Biotech | each | EUR 1.2 |
100 bp DNA Ladder |
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| MBS9143983-05mL | MyBiosource | 0.5mL | EUR 165 |
100 bp DNA Ladder |
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| MBS9143983-25mL | MyBiosource | 2.5mL | EUR 340 |
100 bp DNA Ladder |
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| MBS9143983-5x25mL | MyBiosource | 5x2.5mL | EUR 1345 |
100 bp DNA Ladder |
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| MBS433528-005mg | MyBiosource | 0.05mg | EUR 175 |
100 bp DNA Ladder |
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| MBS433528-5x005mg | MyBiosource | 5x0.05mg | EUR 640 |
100 bp DNA Ladder |
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| MD104-01-50rxns | Vazyme | 50 rxns | EUR 19.3 |
100 bp DNA Ladder |
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| MD104-02-100rxns | Vazyme | 100 rxns | EUR 30.03 |
100 bp DNA Ladder |
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| RK30193 | Abclonal | 500μL | EUR 130.8 |
20 bp DNA Ladder |
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| M-212L | Jena Bioscience GmbH | 5 x 500µl | EUR 346 |
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Description: ready-to-use, orange |
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20 bp DNA Ladder |
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| M-212S | Jena Bioscience GmbH | 500µl | EUR 86.6 |
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Description: ready-to-use, orange |
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50 bp DNA Ladder |
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| M-213L | Jena Bioscience GmbH | 5 x 500µl | EUR 288.4 |
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Description: ready-to-use, orange |
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50 bp DNA Ladder |
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| M-213S | Jena Bioscience GmbH | 500µl | EUR 72.1 |
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Description: ready-to-use, orange |
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50 bp DNA Ladder |
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| DL008 | Geneaid Biotech | each | EUR 45 |
50 bp DNA Ladder |
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| DL008S | Geneaid Biotech | each | EUR 2.2 |
50 bp DNA Ladder |
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| MBS433533-005mg | MyBiosource | 0.05mg | EUR 215 |
50 bp DNA Ladder |
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| MBS433533-5x005mg | MyBiosource | 5x0.05mg | EUR 815 |
Apex DNA Ladder I |
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| 42-427 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 295.59 |
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Description: 200 Lanes, 200-12,000bp |
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Apex DNA Ladder I |
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| 42-428 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 463.43 |
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Description: 500 Lanes, 200-12,000bp |
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Apex DNA Ladder II |
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| 42-429 | Genesee Scientific | 1 x 0.5ml/Unit | EUR 90.91 |
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Description: 100 Lanes, 100-1,000bp |
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Apex DNA Ladder II |
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| 42-430 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 181.82 |
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Description: 200 Lanes, 100-1,000bp |
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Apex DNA Ladder II |
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| 42-431 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 454.32 |
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Description: 500 Lanes, 100-1,000bp |
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XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
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| 300007 | GeneOn | 50µg/500µl | EUR 84 |
XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
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| 300008 | GeneOn | 5x50 µg | EUR 253.2 |
VC 50bp DNA Ladder, 50µg |
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| NL1421 | Vivantis | each | Ask for price |
ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads |
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| ACT-IDMWD2 | ACTGene | each | EUR 178.8 |
Apex DNA Ladder III |
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| 42-424 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 187.26 |
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Description: 200 Lanes, 80bp - 10Kb |
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Apex DNA Ladder III |
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| 42-425 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 467.98 |
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Description: 500 Lanes, 80bp - 10Kb |
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Apex PCR DNA Ladder |
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| 42-432 | Genesee Scientific | 1 x 0.5ml/Unit | EUR 72.48 |
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Description: 100 Lanes, 100-3,000bp |
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Apex PCR DNA Ladder |
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| 42-433 | Genesee Scientific | 1 x 1.0ml/Unit | EUR 144.97 |
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Description: 200 Lanes, 100-3,000bp |
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Apex PCR DNA Ladder |
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| 42-434 | Genesee Scientific | 1 x 2.5ml/Unit | EUR 362.3 |
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Description: 500 Lanes, 100-3,000bp |
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Apex 500bp DNA Ladder |
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| 19-113 | Genesee Scientific | 1ml/Unit | EUR 132.31 |
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Description: 200 Gel Lanes, 500bp - 5kb |
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*Low Range DNA Ladder |
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| IDNALDRLEL500UL | Innovative research | each | EUR 157 |
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Description: *Low Range DNA Ladder |
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