Genome-wide evaluation within the mouse embryo reveals the significance of DNA methylation for transcription integrity
Mouse embryos purchase international DNA methylation of their genome throughout implantation. Nevertheless the precise roles of DNA methyltransferases (DNMTs) in embryos haven’t been studied comprehensively. Right here we systematically analyze the implications of genetic inactivation of Dnmt1, Dnmt3a and Dnmt3b on the methylome and transcriptome of mouse embryos.
We discover a strict division of operate between DNMT1, liable for upkeep methylation, and DNMT3A/B, solely liable for methylation acquisition in improvement. By analyzing severely hypomethylated embryos, we uncover a number of capabilities of DNA methylation that’s used as a mechanism of repression for a panel of genes together with not solely imprinted and germline genes, but additionally lineage-committed genes and 2-cell genes.
DNA methylation additionally suppresses a number of retrotransposons and illegitimate transcripts from cryptic promoters in transposons and gene our bodies. Our work supplies a radical evaluation of the roles of DNA methyltransferases and the significance of DNA methylation for transcriptome integrity in mammalian embryos.
Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
Transcription components (TFs) regulate goal genes by particular interactions with DNA sequences. Detecting and understanding these interactions on the molecular degree is of basic significance in organic and medical contexts. Crosslinking mass spectrometry is a robust software to help the construction prediction of protein complexes however has been restricted to the examine of protein-protein and protein-RNA interactions.
Right here, we current a femtosecond laser-induced crosslinking mass spectrometry (fliX-MS) workflow, which permits the mapping of protein-DNA contacts at single nucleotide and as much as single amino acid decision. Utilized to recombinant histone octamers, NF1, and TBP in complicated with DNA, our methodology is extremely particular for the mapping of DNA binding domains.
Recognized crosslinks are in shut settlement with earlier biochemical information on DNA binding and largely match recognized complicated buildings. Making use of fliX-MS to cells identifies a number of bona fide crosslinks on DNA binding domains, paving the way in which for future massive scale ex vivo experiments.
Recombinant Staphylococcus Aureus sbi Protein (aa 30-437) (strain MSSA476) |
|||
| VAng-Cr6418-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4068 |
|
Description: Staphylococcus Aureus (strain MSSA476) Immunoglobulin-binding protein sbi (sbi), recombinant protein. |
|||
Recombinant Staphylococcus Aureus sbi Protein (aa 30-437) (strain MSSA476) |
|||
| VAng-Cr6418-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2766 |
|
Description: Staphylococcus Aureus (strain MSSA476) Immunoglobulin-binding protein sbi (sbi), recombinant protein. |
|||
Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
|||
| VAng-Cr6433-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4068 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
|||
| VAng-Cr6433-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2914.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus sepF Protein (aa 1-187) (strain MSSA476) |
|||
| VAng-Cr6433-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1990.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein SepF (sepF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
|||
| VAng-Cr6446-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4184.4 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
|||
| VAng-Cr6446-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splB Protein (aa 37-240) (strain MSSA476) |
|||
| VAng-Cr6446-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splB (splB), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
|||
| VAng-Cr6449-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4184.4 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
|||
| VAng-Cr6449-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus splC Protein (aa 37-239) (strain MSSA476) |
|||
| VAng-Cr6449-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
|
Description: Staphylococcus Aureus (strain MSSA476) Serine protease splC (splC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
|||
| VAng-Cr6472-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4167.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
|||
Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
|||
| VAng-Cr6472-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2980.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
|||
Recombinant Staphylococcus Aureus tdk Protein (aa 1-199) (strain MSSA476) |
|||
| VAng-Cr6472-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2023.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Thymidine kinase (tdk), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
|||
| VAng-Cr6497-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3789.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
|||
| VAng-Cr6497-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2716.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureE Protein (aa 1-150) (strain MSSA476) |
|||
| VAng-Cr6497-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1858.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreE (ureE), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
|||
| VAng-Cr6500-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4398 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
|||
| VAng-Cr6500-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3129.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureF Protein (aa 1-229) (strain MSSA476) |
|||
| VAng-Cr6500-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2139.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreF (ureF), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureG Protein (aa 1-204) (strain MSSA476) |
|||
| VAng-Cr6506-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4200 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreG (ureG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureG Protein (aa 1-204) (strain MSSA476) |
|||
| VAng-Cr6506-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreG (ureG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ureG Protein (aa 1-204) (strain MSSA476) |
|||
| VAng-Cr6506-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
|
Description: Staphylococcus Aureus (strain MSSA476) Urease accessory protein UreG (ureG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraC Protein (aa 1-121) (strain MSSA476) |
|||
| VAng-Cr6519-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3574.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraC (vraC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraC Protein (aa 1-121) (strain MSSA476) |
|||
| VAng-Cr6519-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2551.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraC (vraC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraC Protein (aa 1-121) (strain MSSA476) |
|||
| VAng-Cr6519-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1759.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraC (vraC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraX Protein (aa 1-55) (strain MSSA476) |
|||
| VAng-Cr6526-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3096 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraX (vraX), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraX Protein (aa 1-55) (strain MSSA476) |
|||
| VAng-Cr6526-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2205.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraX (vraX), recombinant protein. |
|||
Recombinant Staphylococcus Aureus vraX Protein (aa 1-55) (strain MSSA476) |
|||
| VAng-Cr6526-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1528.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein vraX (vraX), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerC Protein (aa 1-298) (strain MSSA476) |
|||
| VAng-Cr6536-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4910.4 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerC (xerC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerC Protein (aa 1-298) (strain MSSA476) |
|||
| VAng-Cr6536-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3474 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerC (xerC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerC Protein (aa 1-298) (strain MSSA476) |
|||
| VAng-Cr6536-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2386.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerC (xerC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
|||
| VAng-Cr6538-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4893.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
|||
| VAng-Cr6538-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3459.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
|||
Recombinant Staphylococcus Aureus xerD Protein (aa 1-295) (strain MSSA476) |
|||
| VAng-Cr6538-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2370 |
|
Description: Staphylococcus Aureus (strain MSSA476) Tyrosine recombinase XerD (xerD), recombinant protein. |
|||
Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
|||
| VAng-Cr5594-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5602.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
|||
| VAng-Cr5594-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3969.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus acuC Protein (aa 1-389) (strain MSSA476) |
|||
| VAng-Cr5594-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2700 |
|
Description: Staphylococcus Aureus (strain MSSA476) Acetoin utilization protein AcuC (acuC), recombinant protein. |
|||
Recombinant Staphylococcus Aureus araB Protein (aa 1-545) (strain MSSA476) |
|||
| VAng-Cr5613-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) Ribulokinase (araB), recombinant protein. |
|||
Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
|||
| VAng-Cr5668-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5157.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
|||
| VAng-Cr5668-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3657.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus clfA Protein (aa 228-558) (strain MSSA476) |
|||
| VAng-Cr5668-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2502 |
|
Description: Staphylococcus Aureus (strain MSSA476) Clumping factor A (clfA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
|||
| VAng-Cr5684-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3195.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
|||
Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
|||
| VAng-Cr5684-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2271.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
|||
Recombinant Staphylococcus Aureus copZ Protein (aa 1-68) (strain MSSA476) |
|||
| VAng-Cr5684-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1578 |
|
Description: Staphylococcus Aureus (strain MSSA476) Copper chaperone CopZ (copZ), recombinant protein. |
|||
Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
|||
| VAng-Cr5686-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5784 |
|
Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
|||
Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
|||
| VAng-Cr5686-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4084.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
|||
Recombinant Staphylococcus Aureus csd Protein (aa 1-413) (strain MSSA476) |
|||
| VAng-Cr5686-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2782.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Probable cysteine desulfurase (csd), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
|||
| VAng-Cr5693-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3820.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
|||
| VAng-Cr5693-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2733.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ctsR Protein (aa 1-153) (strain MSSA476) |
|||
| VAng-Cr5693-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1875.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Transcriptional regulator CtsR (ctsR), recombinant protein. |
|||
Recombinant Staphylococcus Aureus dnaG Protein (aa 1-605) (strain MSSA476) |
|||
| VAng-Cr5703-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) DNA primase (dnaG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
|||
| VAng-Cr5740-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4926 |
|
Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
|||
Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
|||
| VAng-Cr5740-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3490.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
|||
Recombinant Staphylococcus Aureus era Protein (aa 1-299) (strain MSSA476) |
|||
| VAng-Cr5740-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2386.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) GTPase Era (era), recombinant protein. |
|||
Recombinant Staphylococcus Aureus flp Protein (aa 1-498) (strain MSSA476) |
|||
| VAng-Cr5760-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein flp (flp), recombinant protein. |
|||
Recombinant Staphylococcus Aureus fnbA Protein (aa 1-1015) (strain MSSA476) |
|||
| VAng-Cr5767-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) Fibronectin-binding protein A (fnbA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
|||
| VAng-Cr5775-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6213.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
|||
| VAng-Cr5775-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4398 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ftsA Protein (aa 1-470) (strain MSSA476) |
|||
| VAng-Cr5775-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2997.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Cell division protein FtsA (ftsA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus ftsK Protein (aa 1-789) (strain MSSA476) |
|||
| VAng-Cr5778-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) DNA translocase FtsK (ftsK), recombinant protein. |
|||
Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
|||
| VAng-Cr5784-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4794 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
|||
Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
|||
| VAng-Cr5784-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3393.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
|||
Recombinant Staphylococcus Aureus glcT Protein (aa 1-283) (strain MSSA476) |
|||
| VAng-Cr5784-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2320.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein GlcT (glcT), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
|||
| VAng-Cr5811-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2865.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
|||
| VAng-Cr5811-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2040 |
|
Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hld Protein (aa 1-26) (strain MSSA476) |
|||
| VAng-Cr5811-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1429.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Delta-hemolysin (hld), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
|||
| VAng-Cr5827-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5008.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
|||
| VAng-Cr5827-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3540 |
|
Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus hutG Protein (aa 1-311) (strain MSSA476) |
|||
| VAng-Cr5827-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2419.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Formimidoylglutamase (hutG), recombinant protein. |
|||
Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
|||
| VAng-Cr5848-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4216.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
|||
| VAng-Cr5848-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3013.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus lexA Protein (aa 1-207) (strain MSSA476) |
|||
| VAng-Cr5848-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2056.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) LexA repressor (lexA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus lrgB Protein (aa 1-233) (strain MSSA476) |
|||
| VAng-Cr5869-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Staphylococcus Aureus (strain MSSA476) Antiholin-like protein LrgB (lrgB), recombinant protein. |
|||
Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
|||
| VAng-Cr5910-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4464 |
|
Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
|||
| VAng-Cr5910-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3195.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus mecA Protein (aa 1-239) (strain MSSA476) |
|||
| VAng-Cr5910-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2172 |
|
Description: Staphylococcus Aureus (strain MSSA476) Adapter protein mecA (mecA), recombinant protein. |
|||
Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
|||
| VAng-Cr6005-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3657.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
|||
Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
|||
| VAng-Cr6005-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2617.2 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
|||
Recombinant Staphylococcus Aureus nrdI Protein (aa 1-132) (strain MSSA476) |
|||
| VAng-Cr6005-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1809.6 |
|
Description: Staphylococcus Aureus (strain MSSA476) Protein nrdI (nrdI), recombinant protein. |
|||
Recombinant Staphylococcus Aureus panD Protein (aa 1-24) (strain MSSA476) |
|||
| VAng-Cr6028-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2848.8 |
|
Description: Staphylococcus Aureus (strain MSSA476) Aspartate 1-decarboxylase (panD), recombinant protein. |
|||
The replication equipment of LUCA: frequent origin of DNA replication and transcription
Origin of DNA replication is an enigma as a result of the replicative DNA polymerases (DNAPs) aren’t homologous among the many three domains of life, Micro organism, Archaea, and Eukarya. The homology between the archaeal replicative DNAP (PolD) and the big subunits of the common RNA polymerase (RNAP) liable for transcription suggests a parsimonious evolutionary state of affairs.
Beneath this mannequin, RNAPs and replicative DNAPs developed from a typical ancestor that functioned as an RNA-dependent RNA polymerase within the RNA-protein world that predated the arrival of DNA replication. The replicative DNAP of the Final Common Mobile Ancestor (LUCA) can be the ancestor of the archaeal PolD.
Phosphorylation of mitochondrial transcription issue B2 controls mitochondrial DNA binding and transcription
Mammalian cells comprise genetic data in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression have to be coordinated with nuclear gene expression to reply to mobile energetic wants. To realize perception into the coordination between the nucleus and mitochondria, there’s a want to grasp the regulation of transcription of mitochondrial DNA (mtDNA).
Reversible protein post-translational modifications of the mtDNA transcriptional equipment could also be one solution to management mtDNA transcription. Right here we give attention to a member of the mtDNA transcription initiation complicated, mitochondrial transcription issue B2 (TFB2M). TFB2M melts mtDNA on the promoter to permit the RNA polymerase (POLRMT) to entry the DNA template and provoke transcription.
Three phosphorylation websites have been beforehand recognized on TFB2M by mass spectrometry: threonine 184, serine 197, and threonine 313. Phosphomimetics have been established at these positions. Proteins have been purified and analyzed for his or her capacity to bind mtDNA and provoke transcription in vitro.
Our outcomes point out phosphorylation at threonine 184 and threonine 313 impairs promoter binding and prevents transcription. These findings present a possible regulatory mechanism of mtDNA transcription and assist make clear the significance of protein post-translational modifications in mitochondrial operate.
1 KB DNA LADDER, 500UL PER KIT |
|||
| M-DNA-1KB | CORNING | 1/pk | EUR 84 |
|
Description: Bioscience Mol Bio; DNA Ladder |
|||
ReadiUseâ„¢ 1 Kb Plus DNA Ladder |
|||
| 60050-100ul | AAT Bioquest | 100 ul | EUR 99 |
|
Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
ReadiUseâ„¢ 1 Kb Plus DNA Ladder |
|||
| 60051-2X250ul | AAT Bioquest | 2X250 ul | EUR 203 |
|
Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder |
|||
| 60055-100ul | AAT Bioquest | 100 ul | EUR 79 |
|
Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder |
|||
| 60056-2X250ul | AAT Bioquest | 2X250 ul | EUR 151 |
|
Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
1 kb DNA Ladder in TE buffer |
|||
| 31039 | Biotium | 300uL | EUR 169.2 |
|
Description: Minimum order quantity: 1 unit of 300uL |
|||
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
|||
| AZ-1105-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
50 kb large-Range DNA ladder |
|||
| 308-025 | GeneOn | 250 µg | EUR 144 |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
|||
| AZ-1101 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
|||
| AZ-1105 | Real Time Primers | 500 Lanes | EUR 277.2 |
100bp DNA ladder |
|||
| 9K-004-0002 | Bio Basic | 0.5ml | EUR 260.98 |
100 bp DNA Ladder |
|||
| M1158-500 | Biovision | each | EUR 333.6 |
100 bp DNA Ladder |
|||
| MD104-01 | Vazyme | 250 μl | EUR 132 |
100 bp DNA Ladder |
|||
| MD104-02 | Vazyme | 500 μl | EUR 139.2 |
ACTGene? DNA Marker, 1 kb Ladder, 13 Fragments, 100 loads |
|||
| ACT-IDMWD1 | ACTGene | each | EUR 178.8 |
iVDye 1Kb DNA Ladder |
|||
| V1003-001 | GenDepot | 1ml | EUR 148.8 |
iVDye 1Kb DNA Ladder |
|||
| V1003-025 | GenDepot | 250ul | EUR 91.2 |
iVDye 1Kb DNA Ladder |
|||
| V1003-100 | GenDepot | 4x250ul | EUR 157.2 |
iVDye 1Kb DNA Ladder |
|||
| V1003-250 | GenDepot | 10x250ul | EUR 273.6 |
iVDye 1Kb DNA Ladder |
|||
| V1003-500 | GenDepot | 20x250ul | EUR 457.2 |
OneMark B DNA Ladder (ready-to-use, 250-1000 kb) |
|||
| DM110-0100 | GeneOn | 600 µl | EUR 75.6 |
iVDye 50bp DNA Ladder |
|||
| V1001-001 | GenDepot | 1ml | EUR 218.4 |
iVDye 50bp DNA Ladder |
|||
| V1001-025 | GenDepot | 250ul | EUR 105.6 |
iVDye 50bp DNA Ladder |
|||
| V1001-100 | GenDepot | 4x250ul | EUR 230.4 |
iVDye 50bp DNA Ladder |
|||
| V1001-250 | GenDepot | 10X250ul | EUR 422.4 |
iVDye 100bp DNA Ladder |
|||
| V1002-001 | GenDepot | 1ml | EUR 199.2 |
iVDye 100bp DNA Ladder |
|||
| V1002-025 | GenDepot | 250ul | EUR 96 |
iVDye 100bp DNA Ladder |
|||
| V1002-100 | GenDepot | 4x250ul | EUR 195.6 |
iVDye 100bp DNA Ladder |
|||
| V1002-250 | GenDepot | 10X250ul | EUR 378 |
XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
|||
| 300007 | GeneOn | 50µg/500µl | EUR 84 |
XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes) |
|||
| 300008 | GeneOn | 5x50 µg | EUR 253.2 |
ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads |
|||
| ACT-IDMWD2 | ACTGene | each | EUR 178.8 |
ReadiUseâ„¢ 100 bp DNA Ladder |
|||
| 60070-100ul | AAT Bioquest | 100 ul | EUR 99 |
|
Description: ReadiUseâ„¢ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
ReadiUseâ„¢ 100 bp DNA Ladder |
|||
| 60071-2X250ul | AAT Bioquest | 2X250 ul | EUR 203 |
|
Description: ReadiUseâ„¢ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. |
|||
100 bp Ladder DNA Marker |
|||
| FYD002-500UL | Yeastern Biotech | 30 µg/ 500 µl | Ask for price |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
|||
| 300005 | GeneOn | 86µg/500µl | EUR 84 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
|||
| 300006 | GeneOn | 5x86 µg | EUR 253.2 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
|||
| 300006L | GeneOn | 10x86 µg | EUR 465.6 |
One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye) |
|||
| 300006XL | GeneOn | 20x86 µg | EUR 894 |
Star 1kb DNA Ladder Express |
|||
| BT10701 | Bioatlas | 500µl | EUR 96 |
|
Description: High purity buffer for various PCR applications. |
|||
50 bp Ladder DNA Marker |
|||
| FYD004-500UL | Yeastern Biotech | 32.5 µg/ 500 µl | Ask for price |
100 bp DNA Ladder 500ul per k |
|||
| AXY1014 | Scientific Laboratory Supplies | EACH | EUR 158.4 |
100 BP DNA LADDER, 500UL PER KIT |
|||
| M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
|
Description: Bioscience Mol Bio; DNA Ladder |
|||
Wide range 100 bp DNA Ladder |
|||
| M1192-500 | Biovision | each | EUR 333.6 |
Star 100 bp DNA Ladder Express |
|||
| BA01302 | Bioatlas | 500µl | EUR 96 |
|
Description: High purity buffer for various PCR applications. |
|||
Apoptotic DNA Ladder Isolation Kit |
|||
| K170-50 | Biovision | each | EUR 561.6 |
Apoptotic DNA Ladder Isolation Kit |
|||
| K2045-50 | ApexBio | 50 assays | EUR 602.4 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
|||
| 309-005 | GeneOn | 500 µl | EUR 129.6 |
20 bp TinyOrange DNA Ladder (105ng/µl) |
|||
| 309-025 | GeneOn | 5x500µl | EUR 387.6 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
|||
| AZ-1125-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
|||
| AZ-1135-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
|||
| AZ-1155-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
|||
| AZ-1121 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
|||
| AZ-1125 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
|||
| AZ-1131 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
|||
| AZ-1135 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
|||
| AZ-1151 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
|||
| AZ-1155 | Real Time Primers | 500 Lanes | EUR 277.2 |
1000bp/1kb DNA ladder (no loading dye) |
|||
| 305-005 | GeneOn | 50µg/250µl | EUR 60 |
1000bp/1kb DNA ladder (no loading dye) |
|||
| 305-025 | GeneOn | 5x50µg | EUR 144 |
Quick Apoptotic DNA Ladder Detection Kit |
|||
| K120-50 | Biovision | each | EUR 548.4 |
Quick Apoptotic DNA Ladder Detection Kit |
|||
| K2194-50 | ApexBio | 50 assays | EUR 568.8 |
1Kb Plus DNA Ladder, Ready-to-use |
|||
| 9K-004-0001 | Bio Basic | 0.5ml | EUR 193.98 |
Enhanced Apoptotic DNA Ladder Detection Kit |
|||
| K130-50 | Biovision | each | EUR 529.2 |
Enhanced Apoptotic DNA Ladder Detection Kit |
|||
| K2202-50 | ApexBio | 50 assays | EUR 568.8 |
100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye) |
|||
| W0636-100 | 101Bio | - | Ask for price |
Accuris SmartCheck™ 1kb DNA Ladder, 500ul / 100 lanes |
|||
| PR4100-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
Accuris SmartCheck™ 50bp DNA Ladder, 500ul / 100 lanes |
|||
| PR4005-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
Accuris SmartCheck™ 100bp DNA Ladder, 500ul / 100 lanes |
|||
| PR4010-100 | Benchmark Scientific | 1 PC | EUR 40.3 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
|||
| 305-105 | GeneOn | 50µg/500µl | EUR 60 |
1000bp/1kb DNA ladder (blue, ready-to-use) |
|||
| 305-105L | GeneOn | 10x50 µg | Ask for price |
1000bp/1kb DNA ladder (blue, ready-to-use) |
|||
| 305-105XL | GeneOn | 20x50 µg | Ask for price |
1000bp/1kb DNA ladder (blue, ready-to-use) |
|||
| 305-125 | GeneOn | 5x50 µg | EUR 144 |
100bp plus DNA ladder (blue, ready-to-use) |
|||
| 304-105 | GeneOn | 50µg/500µl | EUR 60 |
100bp plus DNA ladder (blue, ready-to-use) |
|||
| 304-125 | GeneOn | 5x50 µg | EUR 144 |
50bp DNA ladder (no loading dye) (50bp - 1000 bp) |
|||
| 300009 | GeneOn | 50µg/250µl | EUR 60 |
50bp DNA ladder (no loading dye) (50bp - 1000 bp) |
|||
| 300010 | GeneOn | 5x50 µg | EUR 144 |
50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye) |
|||
| W109-100 | 101Bio | - | Ask for price |
OneMark 100 DNA Ladder (ready-to-use, 100-3000 bp) |
|||
| DM101-0100 | GeneOn | 600 µl | EUR 75.6 |
Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500ul / 500 lanes |
|||
| PR4100-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500ul / 500 lanes |
|||
| PR4005-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500ul / 500 lanes |
|||
| PR4010-500 | Benchmark Scientific | 1 PC | EUR 180.4 |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
|||
| 307-105 | GeneOn | 50µg/500µl | EUR 60 |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
|||
| 307-105L | GeneOn | 10x50 µg | Ask for price |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
|||
| 307-105XL | GeneOn | 20x50 µg | Ask for price |
100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use) |
|||
| 307-125 | GeneOn | 5x50µg | EUR 144 |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
|||
| 300003 | GeneOn | 50µg/500µl | EUR 84 |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
|||
| 300003L | GeneOn | 10x50 µg | Ask for price |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
|||
| 300003XL | GeneOn | 20x50 µg | Ask for price |
50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes) |
|||
| 300004 | GeneOn | 5x50 µg | EUR 253.2 |
ACTGene? DNA Marker, 100 bp Ladder, 11 Fragments, 100 loads |
|||
| ACT-IDMWD100 | ACTGene | each | EUR 178.8 |
100 bp DNA Ladder Plus, ready-to-use, 50 ug, 0.5 ug per lane |
|||
| Z6030001-1 | Biochain | 100 lanes | EUR 56 |
100 bp DNA Ladder Plus, ready-to-use, 250 ug, 0.5 ug per lane |
|||
| Z6030001-3 | Biochain | 500 lanes | EUR 224 |
100 bp DNA Ladder Plus, loading dye included, 50 ug, 0.5 ug per lane |
|||
| Z6030001-2 | Biochain | 100 lanes | EUR 50 |